TR OPTIMAL CONDITIONS FOR β-MANNANASE PRODUCTION BY BACILLUS CIRCULANS NT 6.7
نویسندگان
چکیده
منابع مشابه
Cloning, secretory expression and characterization of recombinant β-mannanase from Bacillus circulans NT 6.7
The mannanase gene of B. circulans NT 6.7 was cloned and expressed in an Escherichia coli expression system. The B. circulans NT 6.7 mannanase gene consists of 1,083 nucleotides encoding a 360-amino acid residue long polypeptide, belonging to glycoside hydrolase family 26. The full-length mannanase gene including its native signal sequence was cloned into the vector pET21d and expressed in E. c...
متن کاملCharacterization of mannanase from Bacillus circulans NT 6.7 and its application in mannooligosaccharides preparation as prebiotic.
This study focused on the characterization of mannanase from Bacillus circulans NT 6.7 for mannooligosaccharides (MOS) production. The enzyme from B. circulans NT 6.7 was produced using defatted copra meal as a carbon source. The mannanase was purified by ultrafiltration and column chromatography of Q-Sepharose. The purified protein (M1) was a dimeric protein with a 40 kDa subunit. The purified...
متن کاملExpression of mannanase gene from Bacillus circulans NT 6.7 in Escherichia coli and Lactobacillus plantarum
The mannanase gene of B. circulans NT 6.7 was cloned and expressed in Escerichia coli and food-grade Lactobacillus plantarum expression system. B. circulans NT 6.7 mannanase gene consisted of 1,083 nucleotides encoding 360 amino acid residues which belonging to glycosyl hydrolase family 26. In E. coli system, mannanase gene was cloned into pET21d and expressed in E. coli BL21* (DE3). Beta-manna...
متن کاملOptimisation of batch culture conditions for cyclodextrin glucanotransferase production from Bacillus circulans DF 9R
BACKGROUND: The extracellular enzyme cyclodextrin glucanotransferase (CGTase) synthesizes cyclic malto-oligosaccharides called cyclodextrins (CDs) from starch and related alpha-1,4-glucans. CGTases are produced by a variety of bacteria, mainly Bacillus species, by submerged culture in complex medium. CGTases differ in the amount and types of CDs produced. In addition, CGTase production is highl...
متن کاملGene cloning, expression, and characterization of a novel beta-mannanase from Bacillus circulans CGMCC 1416.
A DNA fragment containing 2,079 base pairs from Bacillus circulans CGMCC 1416 was cloned using degenerate PCR and inverse PCR. An open reading frame containing 981 bp was identified that encoded 326 amino acids residues, including a putative signal peptide of 31 residues. The deduced amino acid sequence showed the highest identity (68.1%) with endo-beta-1,4-D-mannanase from Bacillus circulans s...
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ژورنال
عنوان ژورنال: Bacterial Empire
سال: 2019
ISSN: 2585-9374
DOI: 10.36547/be.2019.2.2.41-45